What is the proper way to reconstitute DSIP and Epitalon peptides?

Reconstitute each peptide separately by adding 2mL of bacteriostatic water to the DSIP vial and 2mL to the Epitalon vial. Always direct the water down the side of the vial at an angle, not directly onto the powder. Gently swirl (never shake) the vials until the powder is fully dissolved. This creates the proper concentration for each peptide before combining them in the nasal spray bottle.

How long does the reconstituted nasal spray remain stable?

When stored properly in the refrigerator, the reconstituted DSIP and Epitalon nasal spray should remain stable for 30-45 days. Always refrigerate between uses and label the bottle with the date of preparation. Discard any unused solution after 45 days, or sooner if you notice any cloudiness, discoloration, or particles in the solution.

What is the correct dosage and timing for DSIP & Epitalon nasal spray?

The typical dosage is 2-3 sprays per nostril (approximately 0.4-0.6mL total) administered 30-60 minutes before bedtime. Each spray delivers about 0.1mL of the combined peptide solution. Start with a lower dose (2 sprays per nostril) and gradually increase if needed based on your individual response. Always use the nasal spray in the evening to support sleep quality.

Reconstituting DSIP & Epitalon | Making a Nasal Spray for Sleep (Step-by-Step Guide)

Supplies You'll Need

DSIP peptide vial (typically 5mg)
Epitalon peptide vial (typically 10mg)
Bacteriostatic water (BAC water)
3mL syringes with needles
Alcohol swabs
30mL amber nasal spray bottle
Measuring tape or ruler

Step 1: Prepare Your Workspace

Clean your work area thoroughly and wash your hands. Gather all supplies and ensure everything is sterile. Remove caps from both peptide vials and wipe the rubber stoppers with alcohol swabs.

Step 2: Calculate Reconstitution Volume

For DSIP (5mg vial): Add 2mL of bacteriostatic water to create a 2.5mg/mL concentration.

For Epitalon (10mg vial): Add 2mL of bacteriostatic water to create a 5mg/mL concentration.

Step 3: Reconstitute DSIP

Draw 2mL of bacteriostatic water into your syringe. Insert the needle into the DSIP vial at an angle, directing the water down the side of the vial (not directly onto the powder). Let the water run slowly down the glass. Remove the needle and gently swirl (do not shake) until the powder is fully dissolved.

Step 4: Reconstitute Epitalon

Using a fresh syringe, draw 2mL of bacteriostatic water. Insert the needle into the Epitalon vial at an angle, directing the water down the side. Allow the water to slowly run down the glass. Remove the needle and gently swirl until completely dissolved.

Step 5: Prepare the Nasal Spray Bottle

Remove the cap and spray mechanism from your 30mL amber bottle. Add approximately 20mL of bacteriostatic water to the bottle as your base.

Step 6: Add Peptides to Nasal Spray Bottle

Draw up the entire contents of the reconstituted DSIP vial and transfer it into the nasal spray bottle. Then draw up the entire contents of the reconstituted Epitalon vial and add it to the same bottle. This creates a combined solution.

Step 7: Mix and Prime the Spray

Replace the spray mechanism and cap. Gently swirl the bottle to mix thoroughly. Prime the pump by spraying several times until you get a consistent mist. Each spray typically delivers 0.1mL.

Step 8: Dosing Instructions

Typical dosing: 2-3 sprays per nostril before bedtime. This provides approximately 0.4-0.6mL total, delivering a combined dose of both peptides. Start with a lower dose and adjust as needed based on your response.

Step 9: Storage

Store your nasal spray in the refrigerator between uses. The combined solution should remain stable for 30-45 days when refrigerated. Label the bottle with the date of preparation.

Tips for Best Results

Always blow your nose gently before using the spray to clear nasal passages
Tilt your head slightly forward when spraying
Alternate nostrils with each spray
Use the spray 30-60 minutes before bedtime for optimal sleep support
Keep the spray bottle upright when not in use